e click edu cell proliferation flow cytometry assay kit  (Elabscience Biotechnology)

Journal: Breast Cancer Research : BCR

Article Title: BHLHE22, targeted by deubiquitinating enzyme OTUD3, exerts an antitumor role in triple-negative breast cancer progression via transcriptionally inhibiting CDT1

doi: 10.1186/s13058-025-02198-0

Figure Lengend Snippet: mRNA-seq analysis identifies downstream target genes of BHLHE22. a , b The results of 2D and 3D principal component analysis (PCA) of the sequenced samples. c Taking |log2(fold change)|≥ 2 and p value < 0.001 as thresholds, the volcano map showed the differential expression of genes in BHLHE22-overexpressed cells. d The Cluego network diagram showed that the enriched functions of these differentially expressed gene (DEGs). e KEGG enrichment analysis of DEGs was shown by bubble map. f The link and overlapping of associated molecules among proliferation-related terms were presented as Cluego network diagram. g The Venn diagram showed overlapping genes in these four terms. h The expression of overlapping genes in BHLHE22-overexpressed cells and control cells was shown with heat maps

Article Snippet: DNA replication in the cells was detected using the EdU cell proliferation assay kit (cat. E-CK-A370, Elabscience, China).

Techniques: Quantitative Proteomics, Expressing, Control

Journal: Journal of Translational Medicine

Article Title: Sodium-myoinositol cotransporter-1 downstream of m6A methyltransferase WTAP exerts a potential carcinogenicity in diffuse large B-cell lymphoma progression

doi: 10.1186/s12967-025-07303-7

Figure Lengend Snippet: SMIT1 facilitates DLBCL cell proliferation, cell cycle progression and inhibits cell apoptosis in vitro. ( A ) qPCR assays showing the expression of SMIT1 mRNA in DLBCL-U2932 and DLBCL-OCI-Ly10 cells. ( B ) CCK8 cell viability assay was used for assessing DLBCL cell proliferation. ( C and E ) Measurement of DLBCL cell proliferation by Click-iT ® EdU flow cytometry assay. ( D and F ) Flow cytometry-based cell cycle detection and quantification in DLBCL cells. ( G ) Western blot assay showing the expression of cell cycle-related protein cyclin D1 and CDK4 in DLBCL cells. ( H and I ) Flow cytometry-based apoptosis detection and quantification in DLBCL cells. ( J ) Western blot assay showing the expression of apoptosis-related protein Bcl-2 and Bax in DLBCL cells. Data are presented as mean ± SD. † p < 0.05, †† p < 0.01, and ††† p < 0.001

Article Snippet: EdU Cell Proliferation assay was performed using an E-Click plus EdU FITC Flow Cytometry Assay Kit according to the manufacturer’s instructions (Elabscience, Houston, TX, USA, E-CK-A370).

Techniques: In Vitro, Expressing, Viability Assay, Flow Cytometry, Western Blot

Journal: Journal of Translational Medicine

Article Title: Sodium-myoinositol cotransporter-1 downstream of m6A methyltransferase WTAP exerts a potential carcinogenicity in diffuse large B-cell lymphoma progression

doi: 10.1186/s12967-025-07303-7

Figure Lengend Snippet: SMIT1 facilitates DLBCL progression by activating AKT signaling. ( A ) Western blot assay showing the expression levels of phospho-AKT (Ser473) and total AKT in U2932 cells. ( B ) CCK8 cell viability assay at 24 h was used for assessing cell proliferation. ( C and D ) Measurement of proliferative cells by Click-iT ® EdU flow cytometry assay. ( E and F ) Flow cytometry-based apoptosis detection and quantification in U2932 cells. Data are presented as mean ± SD. † p < 0.05, †† p < 0.01, and ††† p < 0.001

Article Snippet: EdU Cell Proliferation assay was performed using an E-Click plus EdU FITC Flow Cytometry Assay Kit according to the manufacturer’s instructions (Elabscience, Houston, TX, USA, E-CK-A370).

Techniques: Western Blot, Expressing, Viability Assay, Flow Cytometry